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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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SNAP-Cell TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag fusion proteins inside living cells, on cell surfaces, or in vitro.
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MW 817.64 g/mol. Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
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Fluorogenic substrate for Cathepsin H (Km=150 µM) and aminopeptidases. Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Purity: ≥99%. Long Term Storage: -20°C.
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A fluorogenic substrate for SIRT1, SIRT2, and SIRT3; AMC is released upon enzymatic cleavage by SIRT1, SIRT2, or SIRT3 and its fluorescence can be used to quantify SIRT1, SIRT2, and SIRT3 activity; ex/em = 340-360/440-460 nm, respectively
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MW 838.7 Da, Purity >97%. Ac-WEHD-AFC is a useful fluorogenic substrate for group I caspases (caspase-1, -4, and -5). Caspase activity can be quantified by fluorescent detection of free AFC that has an excitation maximum at 400 nm and emission maximum at 505 nm.
Colorimetric granzyme B substrate. Combinatorial and kinetic studies have found the IEPD sequence to be optimal for cleavage of a tetrapeptide substrate by recombinant granzyme B, with a Km for Ac-IEPD-pNA of 57 µM. Cleavage of the substrate by granzyme B releases pNA, which can be detected by its absorbance at 405 nm. Alternative name: Caspase 8 substrate (chromogenic), Granzyme B substrate (chromogenic). Purity: ≥95%. Solubility: Soluble to at least 100mM in DMSO. Long Term Storage: -20°C.
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The GS peptide sequence (PLSRTLSVSS) is derived from an N-terminus of glycogen synthase and is suitable for use as the substrate for DCAMKL1 and CAMKII family.
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